Campus Directory: Roy Golsteyn

University of Lethbridge

Roy Golsteyn
Faculty
Biological Sciences Department
Office: D870 (University Hall)
Phone: (403) 332-4553
Email:

Degrees

Ph.D. (Biochemistry), University of Cambridge
MSc, Medical Biochemistry, University of Calgary
BSc, Biological Sciences, University of Lethbridge

Expertise

Cancer Research, Cell division, DNA damage, Pharmaceutical industry

Research Areas

Understanding how cancer cells divide, Checkpoint adaptation

Previous Research Areas

Drug discovery, Cancer research, Small molecules, Cell cycle, Cell motility

Alternate Languages

French

Office Hours

by appointment 1:00 PM to 5:00 PM

About Me

Courses

Biol 3105 Cell Signalling
Biol 3115 Cell Growth

Biography

PhD Biochemistry University of Cambridge

Senior Scientist in Cancer Drug Discovery
The Servier Pharmaceutical Company, Paris, France

Selected Publications

1. Lewis, C.W. and R.M. Golsteyn. Cancer cells that survive checkpoint adaptation contain micronuclei that harbour damaged DNA. Cell Cycle. In press 2016.

2. Swift, L.H. and R.M. Golsteyn. Cytotoxic amounts of cisplatin induce either checkpoint adaptation or apoptosis in a concentration dependent manner in cancer cells. Biology of the Cell. 108:127-148. 2016.

3. Swift, L.H. and R.M. Golsteyn. "The relationship between checkpoint adaptation and mitotic catastrophe in genomic changes in cancer cells." In Genome Stability. Ed. Kovalchuk, I. and Kovalchuk, O. Elsevier, 2016

4. Golsteyn, R.M. Cyclin Dependent Kinases (Cdks), Encyclopedia of Cancer, Springer, M. Schwab. Ed. 3rd Edition 2015.

5. Lewis, C.W., R.G. Taylor, and R.M. Golsteyn. "Measurement of Cdk1/cyclin B kinase activity by specific antibodies and western blotting." Methods in Molecular Biology. 1342:337-348. 2015.

6. Kernéis, S., L.H. Swift, C.W. Lewis, C. Bruyère, N. Oumata, P. Colas, S. Ruchaud, J. Bain and R.M. Golsteyn. "Natural product extracts of the Canadian prairie plant, Thermopsis rhombifolia, have anti-cancer activity in phenotypic cell based assays." Natural Product Research. 29:1026-1034. 2015.

7. Swift, L.H. and R.M. Golsteyn. "Genotoxic anti-cancer agents and their relationship to DNA damage, mitosis, and checkpoint adaptation in proliferating cancer cells." International Journal of Molecular Sciences 15:3403-3431. 2014.

8. Lewis, C.W., R.G. Taylor, P.M. Kubara, K. Marshall, L. Meijer, and R.M. Golsteyn. "A western blot assay to measure cyclin dependent kinase activity in cells or in vitro without the use of radioisotopes." FEBS Letters 587:3089-3095. 2013.

9. Kubara, P.M., S. Kernéis, A. Studeny, B. Lanser, L. Meijer and R.M. Golsteyn. "Human cells enter mitosis with damaged DNA after treatment with pharmacological concentrations of genotoxic agents." Biochemical Journal, 446:373-381. 2012.

10. Perron-Sierra, F.M., N. Kucharkzyk, C. Boucley, C. Guyard-Daumas, S. Sciberras, C. Fouache, A. Studény, C. Bossard, P.J. Casara and R.M. Golsteyn. "Synthesis of cis-fused pyran indolocarbazole derivatives that inhibit FLT3 kinase and the DNA Damage Kinase, checkpoint kinase 1." Anti-Cancer Agents and Medicinal Chemistry 12:194-201. 2012.

11. Ferry, G., A. Studény, C. Bossard, P.M. Kubara, D. Zeyer, J.-P. Renaud, P. Casara, G. de Nanteuil, M. Wierzbicki, B. Pfeiffer, M. Prudhomme, S. Leonce, A. Pierré, J.A. Boutin, and R.M. Golsteyn. "Identification and characterization of novel Checkpoint kinase 1 inhibitors by in vitro and cell based screening." Life Sciences. 89:259-268. 2011.



In The Media

1. Leap -Alberta Cancer Foundation. "University of Lethbridge Bean Counters- Buffalo Bean" Fall 2015.
2. The Lethbridge Herald. "U of L research projects receive $200k in funding." 7 August 2015.
3. Western Producer (newspaper). "Cancer researcher plucking wild flowers." 2 July 2015
4. Summer 2015 (Country-guide.ca; Manitoba cooperator.ca; Grain News.ca) "Buffalo bean more than floral for cancer researchers." Lisa Guenther. 6 June 2015.
5. Radio "CJOC 94.7" and 98.1 FM. Pat Siedlecki. U of L researchers discover buffalo bean may have anti-cancer properties. 03 June 2015.
6. Radio "Country 95" FM. Patrick Burles. Southern Alberta plant could become cancer fighter according to U of L researchers. 02 June 2015.
7. QR77 Alberta News. "Out of this world technology to be transferred to the medical field". Peter Watts. Radio. 19 April 2013.
8. "The Tomorrow Cancer Project" Lethbridge College News. 12 February 2013.

Research Interests

The Cancer Cell Laboratory focuses on two projects:

1. The Prairie to Pharmacy Project. We are investigating plant species from the prairie ecological zone for novel anti-cancer compounds. This is a multidisciplinary project that includes collaborators with international pharmaceutical companies.


2. Checkpoint Adaptation project. We examine how cancer cells respond to cancer treatments. We are testing the hypothesis that cancer cells undergo a pathway known as "checkpoint adaptation" (entering mitosis with damaged DNA). We use cultured human cells and cell biological techniques such as microscopy, RNA interference, transfection and Western blotting.


Interested students and recent PhD graduates are invited to apply for research positions.

Current Research and Creative Activity

TitleLocationGrant InformationPrincipal InvestigatorCo Researchers
A new direction in cancer research: Testing the role of epigenetics in failed cell division Lethbridge, AB University of Lethbridge Research Fund, 2010-2011.

Roy Golsteyn
An inverted light microscope to observe human cancer cells Lethbridge, AB Alberta Cancer Foundation, 2010-2011.

Roy Golsteyn
Associate Professor, Lethbridge, AB, Canada Lethbridge, AB Roy Golsteyn
Checkpoint adaptation: a molecular analysis of how human cells respond to genotoxic events Lethbridge, AB Alberta Ingenuity/Innovation, 2010-2013.

Roy Golsteyn

Previous Research

TitleGrant AgencyCompletion Date
Characterization of Checkpoint adaptation in human cancer cells Alberta Cancer Board 2008-2010 2010
Mitotic catastrophe in human cancer cells University of Lethbridge Start-up funds 2007-2009 2009
Mitotic catastrophe, CNRS French Embassy of Canada, 2008. 2008
Inhibitors of protein kinases Institut de Recherches Servier 2007
Role of Cdc25C phosphorylation in DNA damage CIFRE (Conventions Industrielles de Formation par la Recherche, France) 2007


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