- Agricultural Biotechnology
- Office: D882
- Phone: (403) 329-2309
- Fax: (403) 329-2082
- Phone: (403) 329-2309
ExpertiseMolecular Microbiology, Hydrolytic Enzymes, Intestinal Microbiology, Microbial Ecology, Gene expression
Research AreasPhytases produced by ruminal microorganisms, Microbe-insect interactions, Microbial control agents, Genetics of fibre digestion, Microbial ecology of mammalian digestive tract, Water and livestock waste
Cellular Basis of Life (Biology 1010)
Principles of Microbiology (Biology 3400)
Agricultural and Environmental Microbiology
Advances in Agricultural Biotechnology (Biology 4100)
Selected PublicationsKlima, C.L., T.W. Alexander, R.R. Read, S.P. Gow, C.W. Booker, S. Hannon, C. Sheedy, T.A. McAllister, L.B. Selinger. 2011. Genetic characterization and antimicrobial susceptibility of Mannheimia haemolytica isolated from the nasopharynx of feedlot cattle. Vet. Microbiol. 149:390-398.
Qi, M., P. Wang, N. O'Toole, P.S. Barboza, E. Ungerfeld, M.- B. Leigh, L.B. Selinger , G. Butler, A. Tsang, T.A. McAllister, and R.J. Forster. 2011. Snapshot of the eukaryotic gene expression in muskoxen rumen - A metatranscriptomic approach. PLoS ONE 6(5):e20521.
Qi, M. P. Wang, L. Selinger, L. Yanke, R.J. Forster, T. McAllister. 2011. Isolation and characterization of a ferulic acid esterase (Fae1A) from the rumen fungus Anaeromyces mucronatus. J. Appl. Microbiol. 110:1341-13504.
Thomas, M.C., D. K. Thomas, L. B. Selinger and G. Douglas Inglis. 2011. A new method for in silico design and assessment of 16S rDNA primers for molecular microbial ecology. FEMS Microbiol. Lett. 320(2):152-159.
Lysyk, T. J., L. D. Kalischuk-Tymensen, K. Rochon, and L. B. Selinger. 2010. Activity of Bacillus thuringiensis isolates against immature horn fly and stable fly (Diptera: Muscidae). J. Econ. Entomol. 103:1019-1029.
Abnous, K., S.P.J. Brooks, J. Kwan, F. Matias, J. Green-Johnson, L. B. Selinger, M. Thomas and M. Kalmokoff. 2009. Diets enriched in oat bran or wheat bran temporally and differentially alter the composition of the fecal community of the rat. J. Nutr. 139:2024 - 2031.
Costa, E., N. Puhl, L. B. Selinger, G. D. Inglis. 2009. Characterization of mucosa-associated bacterial communities of the mouse intestine by terminal restriction fragment length polymorphism: utility of sampling strategies and methods to reduce single-stranded DNA artifacts. J. Microbiol. Methods. 78:175-180.
Gruninger, R.J., L.B. Selinger, and S.C. Mosimann. 2009. Structural analysis of a multifunctional, tandemly repeated inositol polyphosphatases. J. Mol. Biol. 392:75-86.
Kalmokoff, M.L., J.W. Austin, T. Cyr, M.A. Hefford, R.M. Teather and L.B. Selinger. 2009. Physical and genetic characterization of an outer membrane protein (OmpM1) containing an N-terminal S-layer-like homology domain from the phylogenetically Gram-positive gut anaerobe Mitsuokella multacida. Anaerobe 15:74-81.
Lowe, R. D. Baines, B. Selinger, J. Thomas, T. McAllister and R. Sharma. 2009. Escherichia coli O157:H7 strain origin, lineage and Shiga toxin 2 expression affect colonization of cattle. Appl. Environ. Microbiol. 75:5074-5081.
Puhl, A. A., R. Greiner, and L. B. Selinger. 2008. Kinetics, substrate specificity, and stereospecificity of two new protein tyrosine phosphatase-like inositol polyphosphatases from Selenomonas lacticifex. Biochem. Cell Biol. 86:322-330.
Puhl, A. A., R. Greiner, and L. B. Selinger. 2008. A protein tyrosine phosphatase-like inositol polyphosphatase from Selenomonas ruminantium subsp. lactilytica has specificity for the 5-phosphate of myo-inositol hexakisphosphate. Intl J Biochem & Cell Biol. 40:2053-2064.
Gruninger, R.G., L. B. Selinger, S. C. Mosimann. 2008. Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase like phytase. PhyAsr. FEBS J. 275:3783-3792.
Geddes, J., F. Eudes, A. Laroche, and L.B. Selinger. 2008. Differential expression of proteins in response to the interaction between the pathogen Fusarium graminearum and its host, Hordeum vulgar. Proteomics 8:545-554.
Puhl, A.A., R.J. Gruninger, R. Greiner, T.W. Janzen, S. C. Mosimann and L.B. Selinger. 2007. Kinetic and structural analysis of a bacterial protein tyrosine phosphatase-like myo-inositol polyphosphatase. Prot. Sci. 16:1368-1378.
Nakashima, B.A., T.A. McAllister, R. Sharma, and L.B. Selinger. 2007. Diversity of phytases in the rumen. Microbial Ecol. 53:82-88.
Rochon, K., T. J. Lysyk, and L. B. Selinger. 2005. Retention of Escherichia coli by House Fly and Stable Fly (Diptera: Muscidae) During Pupal Metamorphosis and Eclosion. J. Med. Entomol. 42:397-403.
Chu, H.-M., R.-T. Guo, T.-W Lin, C.-C. Chou, H.-L. Shr, H.-L. Lai, T.-Y. Tang, K.-J. Cheng, L. B. Selinger, and A. H.-J. Wang, 2004. Structures of Selenomonas ruminantium phytase in complex with persulfated phytate: DSP phytase fold and mechanism for sequential substrate hydrolysis. Structure 12:2015-2024.
Rochon, K. , T. J. Lysyk, and L. B. Selinger. 2004. Persistence of Escherichia coli in the guts of immature house fly and stable fly (Diptera: Muscidae) in relation to larval growth and development. J. Med Entomol. 41:1082-1089.
Strauss C.E. T.A. McAllister, and L.B. Selinger. 2004. Development of Pichia pastoris as a rumen escape vehicle for the intestinal delivery of bioactive peptides. Can J Anim. Sci. 84:611-619.
Research InterestsResearch Interests
My research program is focused on microbial activities and applications in agroecosystems, including enhancement of livestock digestion using microbial derived hydrolytic enzymes, development of microbial communities in the gastrointestinal tract, water and biosolids (e.g., compost and manure) and interactions of microbes with ruminant animals and arthropods. Projects currently underway include i) the characterization of a novel family of phytate degrading enzymes related to protein tyrosine phosphatases, ii) the effect of dietary fibre on gastrointestinal tract microbial communities, iii) the ecoenzymology of prion digestion in compost, iv) the prevalence and diversity of campylobacters in local surface waters and v) microbial antibiotic resistance in feedlot cattle.
Other Research Topics:
Biocontrol agents for cattle ectoparasites
Genetics of fibre digestion by ruminal microorganisms
Microflora of cattle manure
Determination of factors affecting diapause in horn fly